Chambers won in 24.41 seconds, edging Levy by .08 seconds. Both times nudged out Levy's previous NAIA top time of 24.54.
OBU also saw improved and new qualifying times in several other events.
Hannah Helker won the mile with the fifth-best time in the NAIA at 5:08.87, moving her out of provisional status and onto an automatic qualifier.
Chambers and Helker were 1-2 in the 600 meters at 1:36.65 and 1:36.94. Both are new qualifiers in the event - Chambers with the No. 5 time and Helker No. 7, nationally.
Nikita Tracey won the 800 meters and is a new qualifier with the NAIA's No. 3 time at 2:15.32.
Aspen Marical and Cherish Hays went 1-2 in the 60 meter hurdles. Marical's time of 8.98 was an automatic qualifying standard, but she already had a better time in the event and is ranked sixth. Hays, however, is a new provisional qualifier at 9.23.
Levy, ranked No. 1 in the 60 meters, had a qualifying mark of 7.57 but has a better time on the books this season.
Stacy Warrior won the 400 meters in 56.94, but is third nationally with a better time in the event.
Evan Shimanek and Erinn Shaugnessey were 1-2 in the pole vault at 12-9.5 and 11-9, respectively. Shaimenk has a better height on record, while Shaughnessey improved to No. 6 in the NAIA with an automatic berth.
On the men's side, Travis Hinton, Roman Gray and Travis Bish went 1-2-3 in the 600 meters. Hinton improved to No. 10 in the NAIA at 1:21.36, while Gray made the provisional list for the first time at No. 15 in 1:220.07. Bish also improved on his previous provisional time, coming in at 1:22.74.
Cordell Baker posted the No. 9 time in the 3000 at 8:31.70 for the automatic qualifying standard.
In the men's 60 meter hurdles, Airick Johnson improved to ninth in the nation with a provisional standard, 8.27 seconds.
Seagram Kern moved from provisional to automatic qualifier status in the 400 meters with his No. 9 national time of 49.16 seconds.
OBU returns to action Friday at the Nebraska Tune-Up.